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These symptoms, which include irritability, anxiety, difficulty concentrating, increased appetite, restlessness, depression, and insomnia, are classified as affective, somatic, or cognitive by the Diagnostic and Statistical Manual of Mental Disorders (DSM-5)

To determine the ideal sample size n for this study, Yamanes simplified sampling formula was used 18 : where, Z=1.96, was employed, assuming an unknown population proportion p set at 50% with a margin of error d set at 3.5%, resulting in an approximate requirement of 294 participants

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However it has been previously shown that the animal LD50 is closely related to fatal drug toxicity in humans 60

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